The data was analyzed using Bird and Forrester formula. Cytotoxicity test was done using BHK21 cells. 21 samples were used and classified into 3 groups: control, 5% tetracycline hydrochloride and 15% EDTA groups. The purpose of this laboratory experimental study was to determine cytotoxicity differences between 5% tetracycline hydrochloride and 15% EDTA. An effective root canal irrigation solution must be able to dissolve organic and anorganic debris, lubricate endodontic instruments, disinfect microorganism and non toxic. Differences in cytotoxicity between 5% tetracycline hydrochloride and 15% EDTA as root canal irrigant Devi Eka Juniarti, Karlina Samadi, and Achmad Sudirman Department of Conservative Dentistry Faculty of Dentistry, Airlangga University Surabaya - Indonesiaĥ% tetracycline hydrochloride and 15% EDTA as a root canal irrigant have been proven to be able to remove smear layer, open dentinal tubules and have antimicrobial activity.
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